اصلاح الکترود خمیرکربن با نانو ذرات SiO2 و کاربرد آن به عنوان زیست حسگر الکتروشیمیایی در بررسی برهم¬کنش ساختار DNA¬-i-motif با تاموکسیفن و اندازه¬گیری الکتروشیمیایی آن- قسمت 16
Abstract Curved particle blue background

اصلاح الکترود خمیرکربن با نانو ذرات SiO2 و کاربرد آن به عنوان زیست حسگر الکتروشیمیایی در بررسی برهم¬کنش ساختار DNA¬-i-motif با تاموکسیفن و اندازه¬گیری الکتروشیمیایی آن- قسمت 16

Abstract
Telomeres are the protective DNA-protein complexes that play an important role in cellular ageing and cancer. Telomerase enzyme maintains the lengths of chromosomal ends by synthesizing telomeric sequences and involved in around 85% of all cancers. Human telomeres are composed of tandem repeats of a double-stranded DNA sequence, (5-TTAGGG):(5-CCCTAA),Which the cytosine rich sequence can form i-motif structure. It is believed that low pH stabilizes the folded i-motif structure of the C-rich strand, thus providing a competitor for duplex formation relative to the linear C-rich strand at neutral pH, that results in slow hybridization occurring and the formation of duplex. Tamoxifen is widely used as endocrine therapy for estrogen-receptor-positive breast cancer. In the first step of current study, the possibility of electrochemical determination of Tamoxifen was studied on the surface of CPE modified with SiO2 nanoparticle (NSiO2) by DPV and CV methods and the amount of Tamoxifen in real sample was determined using standard addition method.
In the second part of study, the i-motif DNA biosensor was prepared and used for the study of i-motif DNA/Tamoxifen interaction. The electrochemical biosensor was prepared by modifying CPE with NSiOand L-cystein and then DNA immobilization was occurred in this platform and used for i-motif DNA / Tamoxifen interaction. I-motif DNA stabilization is a good strategy for cancer therapy because it can inhabited from telomerase reaction. The interaction between i-motif DNA and Tamoxifen on the electrode surface was evaluated by CV and SW measurement in 100 mM PBS buffer solution (pH 4.50) and also in the presence of [Fe (CN)6]3-. These results demonstrate that after the i-motif DNA deposition on the modified electrode surface, Tam can interact with this structure and its oxidation signal can be seen after the i-motif immobilization and the oxidation signals for Tamoxifen increase by increasing its concentration. Circular dichorism (CD) method was used to make sure about i-motif DNA formation and the mechanism of its interaction. The result showed that this structure was formed at pH 4.5 and its stability reduced by increasing pH. The detection limit for constructed biosensor was 0.06 M.
Keywords: i-motif DNA, Tamoxifen, Anti-cancer, Electrochemical biosensor
Palecek ↑

  • Probe ↑
  • Biosensors ↑
  • Luminescence ↑
  • Surface plasmon resonance
    Deoxyribonucleic acid
    Ribonucleic acid
    5 Macromolecule ↑
  • Accuracy
    Sensivity
    Selectivity
    Nanoparticles ↑
  • Nanometer scale structural geometry ↑
  • Information encodin ↑
  • Self-replicating ↑
  • Self-recognition of structure ↑
  • Self-assembly ↑
  • Nanomaterial ↑
  • Tamoxifen citrate ↑
  • Circular dichroism ↑
  • Working electrode ↑
  • Carbon paste electrodes ↑
  • Nanocomposites ↑
  • Sensors ↑
  • Biosensors ↑
  • Sensing electrode ↑
  • Counter electrode ↑
  • Hydrophobic barrier ↑
  • Substrate ↑

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